Our plugin is an attractive alternative to existing tracing tools because it is fully automated and ready for use within a freely accessible imaging program. We reveal the utility of NeuriteTracer by demonstrating its ability to detect the neurite outgrowth promoting capacity of the rho kinase inhibitor Y-27632. Values obtained by NeuriteTracer correlate strongly with those obtained by semi-manual tracing with NeuronJ and by using a sophisticated analysis package, MetaXpress. After each mouse click, the following image of the temporal stack is activated until the last image is reached or the 'End track' button is pressed. o The tracking is done by clicking on the structure on the image. We find that NeuriteTracer accurately measures neurite outgrowth from cerebellar, DRG and hippocampal neurons. Tracking buttons: o To start recording one track, the user has to click on 'add track'. Given user-defined thresholds, the plugin counts neuronal nuclei, and traces and measures neurite length. The plugin analyzes fluorescence microscopy images of neurites and nuclei of dissociated cultured neurons.
To automate these measurements, we have written NeuriteTracer, a neurite tracing plugin for the freely available image-processing program ImageJ. Here is a quick tutorial for tracking the motion of moving particles (micromotors etc.) for the determination of their speeds (um/s).
#IMAGEJ TRACKING MANUAL#
Generally, these measurements are obtained through labor-intensive manual or semi-manual tracing of images. The quantification of these assays requires accurate measurements of neurite length and neuronal cell numbers in neuronal cultures. If already had CIAtah download Fiji, place in the externalprograms/ Fiji directory/Fiji.app/plugins folder. Functions needed (have entire CIAtah loaded anyways): mmtracking.ijm is the tracking function for use in ImageJ, place in plugins folder.
#IMAGEJ TRACKING UPDATE#
MTrackJ is available from the ImageScience update site. ImageJ+MATLAB based mouse location tracking.
The plugin can handle up to five-dimensional (5D) images of any type supported by ImageJ. In vitro assays to measure neuronal growth are a fundamental tool used by many neurobiologists studying neuronal development and regeneration. MTrackJ is an ImageJ plugin to facilitate motion tracking and analysis.
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